This paper firstly points out the importance of the storage location optimization in Automated Storage and Retrieval Systems (AS/RS). Then it has analyzed the basic situation of the current storage location assignment strategy, and indicated the deficiency of the storage location selection under the current strategy. Afterwards, it has designed the storage location optimization module in AS/RS. And then it has constructed the mathematical model on the storage location assignment in AS/RS, and then optimized and solved it, performed instance simulation design and analysis on the storage location assignment by means of Flexsim. Finally, it has concluded that the storage location assignment model after optimization would solve the storage location assignment problem in AS/RS effectively and improve the loading-unloading efficiency, as will provide technical support for the enterprise to improve efficiency and reduce cost.