Information literacy (IL) forms the basis of lifelong learning and plays very important role for students majoring in science and engineering. As far as higher education concerned, gender differences to may influence students’ academic achievements. In order to evaluate the gender differences on information literacy of undergraduates, we surveyed certain undergraduates using self-made questionnaire. The data of the survey was analyzed by SPSS. The findings of the study indicate that the gap of gender difference does exist in the sphere of information literacy. The result of t-test for two groups of male and female students shows significant difference in terms of mean scores obtained in information consciousness, information competency and information ethics tests.