Biomedical Image-segmentation is one of the ways towards removing an area of attentiveness by making various segments of an image. The segmentation of biomedical images is considered as one of the challenging tasks in many clinical applications due to poor illuminations, intensity inhomogeneity and noise. In this paper, we propose a new segmentation method which is called Optimized K-Means Clustering via Level Set Formulation. The proposed method diversified into two stages for efficient segmentation of soft tissues and tumor’s from MRI brain Scans Images, which is called pre-processing and post-processing. In the first stage, a hybrid approach is considered as pre-processing is called Optimized K-Means Clustering which is the combined approach of Particle Swarm Optimization (PSO) as well as K-Means Clustering for improve the clustering efficiency. We choose the ‘optimal’ cluster centers by Particle Swarm Optimization (PSO) algorithm for improving the clustering efficiency. During the process of pre-processing, these segmentation results suffer from few drawbacks such as outliers, edge and boundary leakage problems. In this regard, post-processing is necessary to minimize the obstacles, so we are implementing pre-processing results by using level-set method for smoothed and accurate segmentation of regions from biomedical images such as MRI brain images over existing level set methods.