Diabetic retinopathy is one of the most serious eye diseases and can lead to permanent blindness if not diagnosed early. The main cause of this is diabetes. Not every diabetic will develop diabetic retinopathy, but the risk of developing diabetes is undeniable. This requires the early diagnosis of Diabetic retinopathy. Segmentation is one of the approaches which is useful for detecting the blood vessels in the retinal image. This paper proposed the three models based on a deep learning approach for recognizing blood vessels from retinal images using region-based segmentation techniques. The proposed model consists of four steps preprocessing, Augmentation, Model training, and Performance measure. The augmented retinal images are fed to the three models for training and finally, get the segmented image. The proposed three models are applied on publically available data set of DRIVE, STARE, and HRF. It is observed that more thin blood vessels are segmented on the retinal image in the HRF dataset using model-3. The performance of proposed three models is compare with other state-of-art-methods of blood vessels segmentation of DRIVE, STARE, and HRF datasets.