Presently considerable amount of work has been done in tele-monitoring which involves the transmission of bio-signals and medical images in the wireless media. Intelligent exchange of bio-signals amongst hospitals needs efficient and reliable transmission. Watermarking adds “ownership” information in multimedia contents to prove the authenticity, to verify signal integrity, or to achieve control over the copy process. This paper proposes a novel session based blind watermarking method with secret key by embedding binary watermark image into (Electrocardiogram) ECG signal. The ECG signal is a sensitive diagnostic tool that is used to detect various cardio-vascular diseases by measuring and recording the electrical activity of the heart in exquisite detail. The first part of this paper proposes a multi-resolution wavelet transform based system for detection ‘P’,‘Q’,‘R’,‘S’,‘T’ peaks complex from original ECG signal of human being. ‘R-R’ time lapse is an important component of the ECG signal that corresponds to the heartbeat of the concerned person. Abrupt increase in height of the ‘R’ wave or changes in the measurement of the ‘R-R’ interval denote various disorders of human heart. Similarly ‘P-P’, ‘Q-Q’, ‘S-S’, ‘T-T’ intervals also correspond to different disorders of heart and their peak amplitude envisages other cardiac diseases. In this proposed method the ‘P Q R S T’-peaks are marked and stored over the entire signal and the time interval between two consecutive ‘R’-peaks and other peaks interval are measured to detect anomalies in behavior of heart, if any. The peaks are achieved by the composition of Daubechies sub-bands wavelet of original ECG signal. The accuracy of the P, QRS and T components detection and interval measurement is achieved with high accuracy by processing and thresholding the original ECG signal. The second part of the paper proposes a Discrete Wavelet Transformation (DWT) and Spread Spectrum based watermarking technique. In this approach, the generated watermarked signal having an acceptable level of imperceptibility and distortion is compared to the Original ECG signal. Finally, a comparative study is done for the intervals of two consecutive ‘R-R’ peaks, ‘P-R’, ‘Q-T’, ‘QTc’, QRS duration, cardiac output between original P, QRS and T components detected ECG signal and the watermarked P,QRS and T components detected ECG signal.